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macs bsa stock solution  (Miltenyi Biotec)


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Bioz Manufacturer Symbol Miltenyi Biotec manufactures this product  
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    Structured Review

    Miltenyi Biotec macs bsa stock solution
    Macs Bsa Stock Solution, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 740 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bsa+solution/MACS+BSA+Stock+Solution/custom%40130-091-376%4042664960
    Average 97 stars, based on 740 article reviews
    macs bsa stock solution - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Tunable Synthetic Hydrogel Modulates Hepatic Lineage Specification of Human Liver Organoid
    Article Snippet: The samples were blocked in the BSA solution (Miltenyi Biotec) for 1 h, incubated with primary antibodies in PBS at room temperature for 2 h, rinsed three times with DPBS, and incubated with secondary antibodies in PBS at room temperature for 1 h. The samples were then rinsed with PBS again and preserved in PBS for imaging. .. HLOs slices were permeabilized with 0.2% Triton X-100 (Sigma–Aldrich) 15 min. After blocking in the BSA solution (Miltenyi Biotec) for 2 h, the samples were incubated with primary antibodies in PBS at 4 °C overnight, then using Tween 20 wash 3 times, and secondary antibodies (Thermo Fisher Scientific) in PBS for 45 min at RT, keep in the dark. .. Finally, Phalloidin (Thermo Fisher Scientific) and Hoechst 33342 (Thermo Fisher Scientific) were used to stain the structure of the organoid and nuclei.

    Incubation:

    Article Title: Tunable Synthetic Hydrogel Modulates Hepatic Lineage Specification of Human Liver Organoid
    Article Snippet: The samples were blocked in the BSA solution (Miltenyi Biotec) for 1 h, incubated with primary antibodies in PBS at room temperature for 2 h, rinsed three times with DPBS, and incubated with secondary antibodies in PBS at room temperature for 1 h. The samples were then rinsed with PBS again and preserved in PBS for imaging. .. HLOs slices were permeabilized with 0.2% Triton X-100 (Sigma–Aldrich) 15 min. After blocking in the BSA solution (Miltenyi Biotec) for 2 h, the samples were incubated with primary antibodies in PBS at 4 °C overnight, then using Tween 20 wash 3 times, and secondary antibodies (Thermo Fisher Scientific) in PBS for 45 min at RT, keep in the dark. .. Finally, Phalloidin (Thermo Fisher Scientific) and Hoechst 33342 (Thermo Fisher Scientific) were used to stain the structure of the organoid and nuclei.

    Article Title: Tunable Synthetic Hydrogel Modulates Hepatic Lineage Specification of Human Liver Organoid
    Article Snippet: Immunofluorescence Analysis: For tri-lineage differentiation samples, differentiated hiPSCs were rinsed once with PBS, fixed in 3.7%PFA in room temperature for 15 min, rinsed three times with DPBS, then permeabilized with 0.5% Triton X-100 (Sigma Aldrich) for 30 min, and rinsed three times with DPBS. .. The samples were blocked in the BSA solution (Miltenyi Biotec) for 1 h, incubated with primary antibodies in PBS at room temperature for 2 h, rinsed three times with DPBS, and incubated with secondary antibodies in PBS at room temperature for 1 h. The samples were then rinsed with PBS again and preserved in PBS for imaging. .. HLOs slices were permeabilized with 0.2% Triton X-100 (Sigma–Aldrich) 15 min. After blocking in the BSA solution (Miltenyi Biotec) for 2 h, the samples were incubated with primary antibodies in PBS at 4 °C overnight, then using Tween 20 wash 3 times, and secondary antibodies (Thermo Fisher Scientific) in PBS for 45 min at RT, keep in the dark.

    Magnetic Cell Separation:

    Article Title: Cell-type-targeted mitochondrial transplantation rescues cell degeneration
    Article Snippet: After 35 days post-induction, cells were dissociated using TrypLE Express (Thermo Fisher Scientific, 12604021), diluted in N2B27 medium, filtered through a 70-μm cell strainer (pluriSelect, 43-50070-51) and centrifuged for 10 min at 300 g at 4 °C. .. The cell pellet was resuspended in MACS buffer (80 μl for 10 7 cells) containing autoMACS Rinsing solution (Miltenyi Biotec, 130-091-222) and 0.5% BSA solution (Miltenyi Biotec, 130-091-376). .. CD90 microbeads (Miltenyi Biotec, 130-096-253) were added to the cells (20 μl for 10 7 cells) and incubated for 15 min on ice.

    Article Title: Cell-type-targeted mitochondrial transplantation rescues cell degeneration.
    Article Snippet: T em urkhan Ayupov, Verónica Moreno-Juan, Serena Curtoni, Alex Fratzl, Upnishad Sharma, Susana Posada-Céspedes, Ramona Ratiu, Rei Morikawa, Alexandra Graff Meyer, Margherita Pezzoli, Glenn Bantug, Morgan Chevalier, Yanyan Hou, Sarah A. Nadeau, Álvaro Herrero-Navarro, Vikram Ayinampudi, Elizabeth Kastanaki, Natasha Whitehead, Rebecca A. Siwicki, Mariana M. Ribeiro, Ji Hoon Han, Annalisa Bucci, Christoph Hess, Simone Picelli, Magdalena Renner, Daniel J. Müller, Cameron S. Cowan, Simon Hansen & Botond Roska1,2 ✉

    Imaging:

    Article Title: Tunable Synthetic Hydrogel Modulates Hepatic Lineage Specification of Human Liver Organoid
    Article Snippet: Immunofluorescence Analysis: For tri-lineage differentiation samples, differentiated hiPSCs were rinsed once with PBS, fixed in 3.7%PFA in room temperature for 15 min, rinsed three times with DPBS, then permeabilized with 0.5% Triton X-100 (Sigma Aldrich) for 30 min, and rinsed three times with DPBS. .. The samples were blocked in the BSA solution (Miltenyi Biotec) for 1 h, incubated with primary antibodies in PBS at room temperature for 2 h, rinsed three times with DPBS, and incubated with secondary antibodies in PBS at room temperature for 1 h. The samples were then rinsed with PBS again and preserved in PBS for imaging. .. HLOs slices were permeabilized with 0.2% Triton X-100 (Sigma–Aldrich) 15 min. After blocking in the BSA solution (Miltenyi Biotec) for 2 h, the samples were incubated with primary antibodies in PBS at 4 °C overnight, then using Tween 20 wash 3 times, and secondary antibodies (Thermo Fisher Scientific) in PBS for 45 min at RT, keep in the dark.



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